GenEZ™ ORF Clone Vectors

Choose from our standard mammalian expression-ready vector or from
over 150 popular commercial vectors for custom cloning

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Overview

GenEZ™ ORF clones offer the highest flexibility in ORF clone customization. Choose from our standard mammalian expression-ready cloning vector or over 150 popular commercial cloning vectors for custom cloning.

Standard Expression Cloning Vector

GenScript's GenEZ™ ORF clones are seamlessly cloned into our standard mammalian expression cloning vector, pcDNA3.1⁺/C-(K)-DYK, using our GenBuilder™ seamless cloning technology. Driven by a CMV promoter, pcDNA3.1⁺/C-(K)-DYK is equipped with a C-terminal DYKDDDDK tag for easy protein detection and purification. GenEZ™ ORF clones are delivered expression and transfection ready, saving researchers valuable time in the lab. There is no additional charge for cloning into pcDNA3.1⁺/C-(K)-DYK.

You can see more detailed vector information & maps.

Vector Selection Guide

Our standard vector pcDNA3.1+/C-(K)-DYK is an expression/transfection ready cloning vector for mammalian systems

pcDNA3.1⁺/C-(K)-DYK
Expression System	Promoter	Bacterial Selection	Copy number	Epitope tag	Length
Mammalian	CMV	Ampicillin	High	C-DYK	5444

Custom Cloning Vectors

For enhanced flexibility, GenEZ™ ORF clones can be directly custom cloned into one of over 150 expression vectors that we offer compatible with mammalian or bacterial systems including pLenti, Gateway pDONR, pcDNA, pET, pPIC, and pUC vectors series.

In addition, for GenEZ™ ORFs cloned into custom vectors can be further customized by the addition of up to 30 bp of 5' or 3' flanking sequence – free of charge.

Only $50 for subcloning
Choose from over 150 popular commercial vectors, download vector list (.xls)
Add up to 30 bp of 5' and 3' flanking sequence for free

ORF Cloning Vectors Maps*

(Click any vector name to download the vector map in PDF)

Mammalian	Yeast	Baculovirus/Insect	Bacterial
pcDNA3.1(+) pcDNA3.1(-) pcDNA3.1(+)_myc-His A pcDNA3.1(+)_myc-His B pcDNA3.1(+)_myc-His C pcDNA3.1(-)_myc-His A pcDNA3.1(-)_myc-His B pcDNA3.1(-)_myc-His C pCI-Neo pcDNA3.1+C-DYK pcDNA3.1+C-HA pcDNA3.1+C-6His pcDNA3.1+C-Myc pcDNA3.1+N-DYK pcDNA3.1+N-HA pcDNA3.1+N-6His pcDNA3.1+N-Myc pcDNA3.1+N-GST(Thrombin) pcDNA3.1+N-GST(TEV) pcDNA3.1/Hygro(+) pcDNA3.1/Hygro(-) pcDNA3.1/Zeo (+) pcDNA3.1/Zeo (-) pCMV-3Tag-1a pCMV-3Tag-2a pCMV-3Tag-3a pCMV-3Tag-4a pcDNA3.1+C-eGFP pcDNA3.1+N-eGFP pcDNA3.1+P2A-eGFP pcDNA3.1+P2A pcDNA3.1+N-DYK-P2A pcDNA3.1+C-DYK-P2A pCMV-3Tag-1a-P2A pCMV-3Tag-3a-P2A pcDNA3.4 pGenLenti	pAO815 pPIC 3.5k pPIC9 pPIC9K pPICZA pPICZB pPICZC pPICZalphaA pPICZalphaB pPICZalphaC pESC-TRP pESC-URA pESC-HIS pESC-LEU	pBacPAK8 pBacPAK9 pAcG2T pAcHLT A pAcHLT B pAcHLT C pAcGHLT A pAcGHLT B pAcGHLT C pAcSG2 pBAC-1 pFastBac1 pFastBacHT-A pFastBacHT-B pFastBacHT-C pFastBac-Dual	pBluescript II KS(-) pBluescript II KS(+) pBluescript II SK(-) pBluescript II SK(+) pET-3a pET-3b pET-3c pET-3d pET-9a pET-11a pET-11b pET-11c pET-11d pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b(+) pET-21a(+) pET-21b(+) pET-21d(+) pET-22b(+) pET-23a(+) pET-24a(+) pET-24a(+)-TEV pET-24b(+) pET-24c(+) pET-24d(+) pET-25b(+) pET-26b(+) pET-27b(+) pET-28a(+) pET-28a(+)-TEV pET-28b(+) pET-28c(+) pET-29a(+) pET-29b(+) pET-29c(+) pET-30a(+) pET-30b(+) pET-30c(+) pET-31b(+) pET-32a(+) pET-32b(+) pET-41a(+) pET-41b(+) pET-41c(+) pET-42a(+) pET-42b(+) pET-42c(+) pET-43.1a(+) pET-43.1b(+) pET-45b(+) pET-50b(+) pET-51b(+) pET-52b(+) pGEX-2TK pGEX-4T-1 pGEX-4T-2 pGEX-4T-3 pGEX-5X-1 pGEX-5X-2 pGEX-5X-3 pGEX-6P-1 pGEX-6P-2 pGEX-6P-3 pMAL-c4x pMAL-c5E pMAL-c5x pMAL-p5E pMAL-p5G pMAL-p5X pQE-1 pQE-60 pGS-21a pETDuet-1 pCDFDuet-1 pRSFDuet-1 pCOLADuet-1 pGEX-4T-1-H(RBS) pGEX-4T-1-M(RBS) pGEX-5X-1-H(RBS) pGEX-5X-1-M(RBS) pGEX-6P-1-H(RBS) pGEX-6P-1-M(RBS) pMAL-c4x-1-H(RBS) pMAL-c4x-1-M(RBS) pGenDONR

*Not all vectors maps shown here. Vectors not shown may need to be shipped by the customer to GenScript for ORF custom cloning.

Search Your Latest Version ORFs at GenScript Now

You can enter the following as search terms:

Entrez Gene ID (e.g. 7157)
gene symbol (e.g. TP53)
gene name (e.g. tumor protein p53)
gene synonyms (e.g. FLJ92943)
Ensembl ID (e.g. ENSG0000141510)

RefSeq Accession (e.g. NM_000546)
Species can be input after the keyword, using format "keyword [species:$species]" where $species can be name of species (like human or rat) or taxon id (like 9606).

Where to target the version number? Example

GenScript